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Objective: To fabricate TiO2 nanotube material functionalized by antimicrobial peptide LL-37, and to explore its effects on biological behaviors such as adhesion and migration of human keratinocytes (HaCaT) and its antibacterial properties. Methods: The TiO2 nanotube array (NT) was constructed on the surface of polished titanium (PT) by anodization, and the antimicrobial peptide LL-37 was loaded on the surface of TiO2 nanotube (LL-37/NT) by physical adsorption. Three samples were selected by simple random sampling in each group. Surface morphology, roughness, hydrophilicity and release characteristics of LL-37 of the samples were analyzed with a field emission scanning electron microscope, an atomic force microscope, a contact angle measuring device and a microplate absorbance reader. HaCaT cells were respectively cultured on the surface of three groups of titanium samples. Each group had 3 replicates. The morphology of cell was observed by field emission scanning electron microscope. The number of cell adhesion was observed by cellular immunofluorescence staining. Cell counting kit-8 (CCK-8) assay was used to detect cell proliferation. Wound scratch assay was used to observe the migration of HaCaT. The above experiments were used to evaluate the effect of each group on the biological behavior of HaCaT cells. To evaluate their antibacterial effects, Porphyromonas gingivalis (Pg) was respectively inoculated on the surface of three groups of titanium samples. Each group had 3 replicates. The morphology of bacteria was observed by field emission scanning electron microscope. Bacterial viability was determined by live/dead bacterial staining. Results: A uniform array of nanotubes could be seen on the surface of titanium samples in LL-37/NT group, and the top of the tube was covered with granular LL-37. Compared with PT group [the roughness was (2.30±0.18) nm, the contact angle was 71.8°±1.7°], the roughness [(20.40±3.10) and (19.10±4.11) nm] and hydrophilicity (the contact angles were 22.4°±3.1° and 25.3°±2.2°, respectively) of titanium samples increased in NT and LL-37/NT group (P<0.001). The results of in vitro release test showed that the release of antimicrobial peptide LL-37 was characterized by early sudden release (1-4 h) and long-term (1-7 d) slow release. With the immunofluorescence, more cell attachment was found on NT and LL-37/NT than that on PT at the first 0.5 and 2.0 h of culture (P<0.05). The results of CCK-8 showed that there was no significant difference in the proliferation of cells among groups at 1, 3 and 5 days after culture. Wound scratch assay showed that compared with PT and NT group, the cell moved fastest on the surface of titanium samples in LL-37/NT group at 24 h of culture [(96.4±4.9)%] (F=35.55, P<0.001). A monolayer cells could be formed and filled with the scratch in 24 h at LL-37/NT group. The results of bacterial test in vitro showed that compared with the PT group, the bacterial morphology in the NT and LL-37/NT groups was significantly wrinkled, and obvious bacterial rupture could be seen on the surface of titanium samples in LL-37/NT group. The results of bacteria staining showed that the green fluorescence intensity of titanium samples in LL-37/NT group was the lowest in all groups (F=66.54,P<0.001). Conclusions: LL-37/NT is beneficial to the adhesion and migration of HaCaT cells and has excellent antibacterial properties, this provides a new strategy for the optimal design of implant neck materials.
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Humans , Titanium/chemistry , Antimicrobial Peptides , Cathelicidins , Sincalide , Anti-Bacterial Agents/pharmacology , Nanotubes/chemistry , Dental Materials , Bacteria , Keratinocytes , Surface PropertiesABSTRACT
miRNA-27a (miR-27a) is a member of miR-23a-27a-24 gene cluster and is abnormally expressed in gastric cancer. miR-27a plays an important role in gastric cancer cell epithelial-mesenchymal transition, proliferation, microenvironment and resistance to chemotherapy drugs by regulating SFRP1, PHLPP2, BTG2, SOCS6, FOXO1 and other tumor suppressor genes. At the same time, single nucleotide polymorphisms of miR-27a and gastric cancer have also become hotspots in recent years. A more comprehensive exploration of the relationship between miR-27a and gastric cancer is expected to provide new methods and ideas in the treatment of gastric cancer. This article reviews the research progress of miR-27a in gastric cancer.
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Hyaluronidase is a type of glycosidase that can specifically degrade hyaluronic acid (HA). There are mainly 6 types, among which hyaluronidase-1 ( HYAL-1) and hyaluronidase-2 ( HYAL-2) has a more in-depth study and has different roles in different tumors. HYAL-1 and HYAL-2 have the potential to be used as biomarkers for the diagnosis of malignant tumors, especially in common malignant tumors such as colorectal cancer, pancreatic cancer, bladder cancer, and prostate cancer. Compared with traditional biomarkers, they have higher specificity and sensitivity. Other studies have shown that Hyaluronidase can assist chemotherapy drugs to enhance the efficacy of drugs. hyaluronidase plays both a role in promoting cancer and a role in suppressing cancer in malignant tumors. This article reviews the mechanism of Hyaluronidase in malignant tumors, which is expected to provide a new method for early diagnosis, early treatment and prognosis of malignant tumors.
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With the expanded application of heavy metal cadmium, soil cadmium pollution is more and more serious. In this study, using Salix matsudana as a phytoremediation candidate, we observed changes of gene expression and metabolic pathway after 1, 7 and 30 days under 2.5 mg/L and 50 mg/L cadmium stress. The result of transcriptome sequencing showed that we obtained 102 595 Unigenes; 26 623 and 32 154 differentially expressed genes (DEG) in the same concentration and different stress time; 8 550, 3 444 and 11 428 DEG with different concentrations at the same time; 25 genes closely related to cadmium stress response were screened. The changes of genes expression (such as metallothionein, ABC transporter, zinc and manganese transporter) depended on both concentration of cadmium and exposure time. The expression of several genes was obviously up-regulated after cadmium stress, for example 3,6-deoxyinosinone ketolase (ROT3) in brassinolide synthesis pathway and flavonoid synthase (FLS), flavanone-3-hydroxylase (F3H) in the synthesis pathway of brassinolide. In addition, GO analysis shows that GO entries were mainly enriched in metabolic processes including cellular processes, membranes, membrane fractions, cells, cellular fractions, catalytic activation and binding proteins in response to cadmium stress, whose number would increase along with cadmium concentration and exposure time. The reliability of transcriptome information was verified by qPCR and physiological experimental data. Response mechanisms of S. matsudana after cadmium stress were analyzed by transcriptome sequencing, which provided theoretical guidance for remediation of cadmium pollution in soil by S. matsudana.
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Biodegradation, Environmental , Cadmium , Toxicity , Gene Expression Profiling , Gene Expression Regulation, Plant , Plant Proteins , Genetics , Reproducibility of Results , Salix , Genetics , Stress, Physiological , Genetics , TranscriptomeABSTRACT
In recent years, driven by high-throughput sequencing technology, it has been found that changes in the proportion of specific microbiota are related to the occurrence and development of pancreatic cancer, including oral microbes, gastrointestinal flora, and pancreatic flora. Porphyromonas has a positive correlation with pancreatic cancer risk, which may provide new biomarkers for the diagnosis of pancreatic cancer. In addition, the microbiota is closely related to various treatments for pancreatic cancer. When the intestinal flora is imbalanced, it will affect the effect of chemotherapy. Bacteria in pancreatic cancer can induce immune tolerance. Complications with bacterial infection can lead to postoperative complications increase.
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MicroRNA-490 (miR-490) is an important member of the miRNA family and is closely related to the occurrence and development of tumors. Studies have shown that miR-490 is abnormally expressed in various tumors such as gastric cancer, colorectal cancer, breast cancer, etc., which plays an oncogene or tumor suppressor gene role in the biological behaviors such as proliferation, apoptosis, invasion and metastasis of different tumor cells. MiR-490 is expected to be an indicator of tumor diagnosis and clinical prognosis, and in-depth study of its mechanism is expected to provide a new direction for the diagnosis and treatment of cancer.
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MicroRNA-490 (miR-490)is an important member of the miRNA family and is closely related to the occurrence and development of tumors. Studies have shown that miR-490 is abnormally expressed in various tumors such as gastric cancer,colorectal cancer,breast cancer,etc. ,which plays an oncogene or tumor suppressor gene role in the biological behaviors such as proliferation,apoptosis,invasion and metastasis of different tumor cells. MiR-490 is expected to be an indicator of tumor diagnosis and clinical prognosis,and in-depth study of its mechanism is expected to provide a new direction for the diagnosis and treatment of cancer.
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Tripartite motif (TRIM) family,a very important protein family,has various functions in cellular biological processes including intracellular signal transduction,cell growth and apoptosis,innate immunity,autophagy and tumorigenesis.The specific expressions of TRIM in hepatocellular carcinoma are proved by many researches,which are closely related to the occurrence,metastasis and prognosis of hepatocellular carcinoma.TRIM are expected to be markers for the occurrence and development of hepatocellular carcinoma,and have broad prospects in the early diagnosis,therapy and prognosis of hepatocellular carcinoma.
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Liquid biopsy is a kind of popular and emerging pathological detection technology,which mainly includes the detection of circulating tumor cells,circulating tumor DNA and exosome.Recent studies show that liquid biopsy not only plays a potential advantage in early diagnosis of pancreatic cancer,but also has some guiding significance for prognosis and recurrence monitoring of pancreatic cancer.
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Objective:To evaluate the impact of the establishing universal basic medical insurance system(UMIS) on pharmaceutical industry in China.Methods:Based on the accumulated main business income data of China's pharmaceutical industry from 2002 to 2014,the influence change of UMIS on China's pharmaceutical industry before and after 2008 was analyzed by the segmented regression model.Results:Segmented regression model showed that the slope rate of development trend of main business income of China's pharmaceutical industry was 1.03×107 thousand yuan per quarter(P=0.000) before the implementation of UMIS in 2008.After the implementation of UMIS in 2008,the slope rate of development trend of main business income of China's pharmaceutical industry was 2.75× 107 thousand yuan per quarter (P=0.000) and trend increased 1.72× 107 thousand yuan per quarter(P=0.000).Conclusion:The UMIS had impact on the structure,sales volume of pharmaceutical products and sale costs of China's pharmaceutical industry.UMIS had a significant effect on the increase of China's pharmaceutical industry.
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Objective To explore the treatment of percutaneous coronary intervention (PCI), and to comparatively study on the protection of side support by using the active and passive expansion technique.Method 78 patients with coronary artery disease were protected by using balloon technique, there were two groups: the active expansion group (n=41) and the side support balloon (n=37), the TIMI classification、myocardial perfusion rating (MBG), TIMI myocardial perfusion rating (TMP) and the incidence of marginal vascular interlayer were observed and analyzed. Results After the main support was put in, the proportion of patients with side branches of TIMI、 MBG and TMP was at level 3 and the proportion of patients with active balloon expansion was 93％, the margin expansion group was 84％, the difference was not statistically significant (P＞0.05); the incidence of intersecting vascular interlayer in active dilatation group was 24％, the margin expansion group was 8％, and there were statistical differences (P＜0.05). Conclusions Using the technique of holding balloon to protect the side branches, both the active expansion of the side support balloon and the expansion of the side support balloon can significantly reduce the risk of the main stent placement in the posterior branch of the blood vessel, however the incidence of side branch vascular interlayer was lower.
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Salt-inducible kinase 1 (SIK1) is closely related to tumorigenesis.The expressions of SIK1 in hepatocellular carcinoma,pancreatic cancer,gastric cancer,lung cancer,ovarian cancer and other tumors are specific,and their abnormal expressions can inhibit or promote the occurrence,development and metastasis of tumor.Abnormal expressions of SIK1 are expected to provide a new method for the diagnosis and treatment of tumors,and provide guidance for clinical prognosis.
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Objective To investigate the clinical value of splenic autotransplantation in patients with severe splenic trauma. Methods A prospective case-control study were performed in 120 patients with traumatic spleen rupture including the treatment group 72 patients and the control group 48 patients. The treatment group were treated with splenectomy plus spleen autotransplantation and the control group merely under splenectomy. Compare the operation time,operative blood loss,postoperative hospital stay,postoperative complications and the immune indexes before and different period after operation. Results Autologous spleen transplantation takes more time than merely splenectomy(P<0.05),but the operative blood loss,postoperative hospital stay and postopera-tive complications were no significant difference. 1 days after operation,the immune indexes of two groups were significantly lower than those before operation(P < 0.05),and 1 week after operation the immune indexes of two groups were significantly elevated(P<0.05).The immune indexes of the treatment group were better than those of the control group 3 months after operation(P < 0.05),and there was no significant difference compared with preoperative. Conclusion Splenectomy cause the decrease in the immune function,but the immune function can quickly rise to a certain level in short term.The splenic autotransplantation can effectively restore the immune func-tion to the preoperative level.
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<p><b>OBJECTIVE</b>To explore the potential decrease of serum erythropoietin (EPO) level in patients with multiple sclerosis (MS) complicated with anemia.</p><p><b>METHODS</b>The serum EPO levels were detected in the patients with MS complicated with anemia (MS group, n=31), patients with iron deficiency anemia (IDA group, n=33), and healthy subjects (normal control group, n=80) by enzyme-linked immunosorbent assay (ELISA). Blood routine test, reticulocyte count, hemoglobin, and indexes of liver and kidney function were also detected.</p><p><b>RESULTS</b>The serum EPO level in MS group was significantly lower than those in IDA group [(101.3±17.6)U/L vs.(166.1±8.7)U/L, P<0.01]. Moreover, the serum EPO level decreased as the severity of anemia in the MS group increased: it was (95.7±9.6), (101.7±8.1), and (123.7±9.3) U/L in patients with mild, moderate, and severe anemia, respectively (P<0.05). Other indicators including blood routine findings, reticulocyte count, hemoglobin, and liver and kidney function parameters showed no significant difference between the MS group and the IDA group (P>0.05).</p><p><b>CONCLUSIONS</b>The serum EPO level decreases in patients with multiple sclerosis complicated with anemia, and the decreasing levels are related with the severity of anemia. Thus EPO therapy may be beneficial for these patients.</p>
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Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Anemia , Blood , Case-Control Studies , Erythropoietin , Blood , Multiple Sclerosis , BloodABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular mechanism of the inhibitory effect of curcumine on the migration and invasion of hepatic stellate cells (HSC).</p><p><b>METHODS</b>Rat hepatic stellate cells were cultured and activated with ConA. Matrix metalloproteinase-2 (MMP-2) expression and activity was determined by Western blot and gelatin zymography. Migration and invasion of HSC was assessed by wound healing assay and modified Boyden chamber assay.</p><p><b>RESULTS</b>Curcumine reduced the level and activity of MMP-2 expression in activated HSC in a dose-dependent manner. When treated with 25, 50 or 100 micromol/L curcumine, the expression of MMP-2 was reduced by 21.8%+/-5.1%, 65.5%+/-9.2% or 87.9%+/-11.5% (P < 0.05), and the activity of MMP-2 was also significantly reduced by curcumine. Migration and invasion of activated HSC was also inhibited by curcumine in a dose-dependent way. When treated with 25, 50 or 100 micromol/L curcumine, the migration of activated HSC was reduced by 27.5%+/-5.8%, 54.4%+/-7.6% or 67.1%+/-9.3% (P < 0.05), and the invasion of activated HSC was also significantly reduced by curcumine.</p><p><b>CONCLUSION</b>Curcumine inhibits migration and invasion of activated HSC by reducing MMP-2 expression and activity.</p>
Subject(s)
Animals , Rats , Blotting, Western , Cell Movement , Cells, Cultured , Concanavalin A , Pharmacology , Curcumin , Pharmacology , Dose-Response Relationship, Drug , Hepatic Stellate Cells , Cell Biology , Metabolism , Liver Cirrhosis , Matrix Metalloproteinase 2 , Metabolism , Tissue Inhibitor of Metalloproteinase-2 , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To determine the effects of berberine hydrochloride on the expressions of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) in periodontal tissues in rat periodontitis model.</p><p><b>METHODS</b>Based on the successful rat periodontitis model, the experimental rats were randomized into different groups followed by oral treatment with berberine hydrochloride for 1, 2, 3, 4 weeks and then the rats were sacrificed and analyzed. Pathological assay and HE staining were used to detect the general conditions and pathological changes of rat periodontal tissues. And immunohistochemical staining was conducted to determine the expressions of IL-1beta and TNF-alpha in rats periodontitis model periodontal tissues.</p><p><b>RESULTS</b>The levels of IL-1beta and TNF-alpha in the periodontitis tissues were significantly higher than that in the control group. Treatment with berberine hydrochloride decreased the levels of IL-1beta and TNF-alpha in periodontitis tissues (P<0.05). Moreover, the general conditions and pathological changes in the control group and groups treated with berberine hydrochloride were much better than that in periodontitis groups.</p><p><b>CONCLUSION</b>Berberine hydrochloride inhibited the expression of IL-1beta and TNF-alpha in periodontal tissues in rats periodontitis model and promoted the regeneration of the periodontal tissues. This study suggested that berberine hydrochloride may have potential clinical application.</p>
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Animals , Male , Rats , Berberine , Interleukin-1beta , Periodontitis , Tumor Necrosis Factor-alphaABSTRACT
<p><b>BACKGROUND</b>Gastrointestinal graft versus host disease (GI-GVHD) and cytomegalovirus (CMV) enteritis are important complications following allogeneic haematopoietic stem cell transplantation (allo-HSCT). We explored the role of colonoscopy in the diagnosis of GI-GVHD and CMV enteritis following allo-HSCT to identify the endoscopic manifestations of GI-GVHD and CMV enteritis was made.</p><p><b>METHODS</b>A retrospective analysis of the colonoscopic manifestations of GI-GVHD, CMV enteritis and GI-GVHD with concurrent CMV enteritis (GconC) and their related clinical issues.</p><p><b>RESULTS</b>Forty-seven patients underwent 50 colonoscopies with diagnoses of 32 GI-GVHD, 7 CMV enteritis and 11 GconC. Both GI-GVHD and CMV enteritis had colonic mucosal lesions with various manifestations under colonoscopy. Tortoise shell like changes of the mucosa (12 of 32) and deep ulcers (2 of 7) were specific endoscopic manifestations for GI-GVHD and CMV enteritis, respectively, while mucosal oedema, erythema, congestion, erosion and shallow ulcers could not be used to differentiate GI-GVHD from CMV enteritis. GconC patients were prone to have oozing bleeding of the end ileal mucosa and typhlodicliditis. Of the biopsed specimens for GI-GVHD, CMV enteritis and GconC, 64%, 70% and 44% were taken from the rectum and sigmoid colon respectively.</p><p><b>CONCLUSIONS</b>Following allo-HSCT, tortoise shell like changes and deep ulcers of the colonic mucosa are characteristic changes for GI-GVHD and CMV enteritis, respectively, while the other lesions are not. Most of the GI-GVHDs and CMV enteritis cases can be diagnosed by left colon examination and tissue biopsy, but total colon examination to the terminal ileum is preferred.</p>
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Adolescent , Adult , Female , Humans , Male , Middle Aged , Colonoscopy , Methods , Cytomegalovirus Infections , Diagnosis , Enteritis , Diagnosis , Graft vs Host Disease , Diagnosis , Hematopoietic Stem Cell Transplantation , Methods , Postoperative Complications , Diagnosis , Reproducibility of Results , Retrospective Studies , Sensitivity and Specificity , Transplantation, HomologousABSTRACT
<p><b>OBJECTIVE</b>To evaluate the feasibility and the results of the procedure of maxillomandibular advancement combined with uvulopalatopharyngoplasty for the treatment of obstructive sleep apnea hypopnea syndrome (OSAHS).</p><p><b>METHODS</b>Nine cases of obese patients with severe OSAHS [age (47.8 +/- 9.7); body mass index (BMI) (35.3 +/- 2.5) kg/m(2); apnea and hypopnea index (AHI) (88.7 +/- 6.7) per hour] underwent the procedure of maxillomandibular advancement (MMA) combined with uvulopalatopharyngoplasty (UPPP). The patients were monitored by polysomnography (PSG) before operation and 3, 6, 12 months after operation, and cephalometric analysis and velopharyngeal closure function were evaluated at the same time.</p><p><b>RESULTS</b>The maxilla was advanced by (8.3 +/- 1.3) mm and the mandible and chin were advanced by (23.0 +/- 2.2) mm. AHI was (2.1 +/- 1.1) per hour post-operation. All patients had no speaking problem and swallowing difficulty and had a good appearance and occlusions. The OSAHS in this group of patients was cured.</p><p><b>CONCLUSIONS</b>Good short-term effects were observed with UPPP and MMA in the treatment of obese patients with severe OSAHS. It did not cause the velopharyngeal closure insufficiency and maxillary necrosis. A long-term follow-up is needed.</p>
Subject(s)
Adult , Humans , Male , Middle Aged , Follow-Up Studies , Mandibular Advancement , Methods , Obesity , General Surgery , Osteotomy, Le Fort , Palate, Soft , General Surgery , Pharyngeal Muscles , General Surgery , Sleep Apnea, Obstructive , General Surgery , Treatment Outcome , Uvula , General SurgeryABSTRACT
<p><b>OBJECTIVE</b>To explore the synergistic protective effect of co-transplanted testicular cells expressing FasL and CsA on survival of islet allografts.</p><p><b>METHODS</b>The allogeneic islets and testicular cells were co-transplanted into the renal subcapsular space of the diabetic recipients with or without CsA after operation. Allografts survival period and the testicular cells or islets function were analyzed.</p><p><b>RESULTS</b>The mean survival period of control group was 4.6 +/- 1.1 days. When CsA was administered after transplantation, the mean survival period of islet allografts, (21.8 +/- 4.7) days, was significantly longer than that of control group (P < 0.01). When islets were co-transplanted together with 1 x 10(7) testicular cells (group A), a significant prolongation of graft survival was found (more than 57.5 +/- 4.0 days; P < 0.01 vs. control). But if 1 x 10(7) testicular cells expressing FasL were cultured with FasL-mAb for 30 minutes before co-transplantation (group B), the mean survival period of islet allografts (5.8 +/- 2.6 days), was similar to that in control group, but significantly shorter than that in group A (P < 0.01). When islets and 1 x 10(5) testicular cells were co-transplanted separately into the bilateral renal subcapsular space with CsA (group C), the survival of islet allografts was significantly prolonged in comparison with control group (more than 55.0 +/- 6.5 days; P < 0.01 vs. control), and similar to islets co-transplanted together with 1 x 10(7) testicular cells (group A). When islets were co-transplanted separately with 1 x 10(6) testicular cells without CsA (group D), the mean survival period (11.5 +/- 3.1 days) was shorter than that in group C, but prolonged in comparison to control group (P < 0.05).</p><p><b>CONCLUSION</b>The co-transplanted testicular cells expressing FasL with administering CsA post-transplantation can jointly inhibit immune rejection of islet allografts by different mechanism and play a systemic and synergistic protective role to islet allografts.</p>
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Animals , Male , Rats , Cyclosporine , Therapeutic Uses , Fas Ligand Protein , Graft Survival , Immunohistochemistry , Insulin , Blood , Islets of Langerhans , Pathology , Islets of Langerhans Transplantation , Membrane Glycoproteins , Genetics , Rats, Sprague-Dawley , Rats, Wistar , Sertoli Cells , Metabolism , Transplantation , Transplantation, HomologousABSTRACT
Objective To explore the immune function injury and its mechanism in drug abuser.Methods The immune function changes in 50 drug abusers were compared with normal healthy populations by detection of the indexes of subgroups of Th cells, transformation rate of lymphocytes,IgA,IgM,IgG,IgE,compliment C3,C4,IL 1, IL 2,IL 6 TNF and NO.Result In peripheral blood the percentage of Th1 cell, transformation rate of lymphocyte, IgA, IgM, IgG, IgE content, compliment C4,C4, IL 1, IL 2,IL 6,and TNF levels were significantly lower than normal(P< 0.01).The value of Th1/Th2 was lower than normal as well(P< 0.05).NO content was significantly higher than normal(P< 0.001).Conclusion The mechanism of immune function injury in drug abuser might be correlative to direct injury of drugs and their inhibition effect on the thymus hypothalamus hypophysis adrenal axis.